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KMID : 0364219840270020093
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Korean Journal of Zoology
1984 Volume.27 No. 2 p.93 ~ p.102
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Experimentally Provoked Double Axes Formation in Xenopus laevis Embryos
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Chung Hae-Moon
Malacinski George M.
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Abstract
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Xenopus laevis eggs were de-jellied and manually manipulated to remove their vitelline membranes. They were then positioned in a variety of orientations re. gravity. That is the future ventral side was located upwards (opposed gravity) or downwards (faced gravity). Development through the tailbud stage was observed and the frequency of double axes formation recorded. Orientation of the egg re. its natural polarity was not an important factor in generating double axes. Its physical structure (flattened re. spherical), however, appeared to be important in determining the frequency of twinning. These observations give insights into the mechsnism of polarity establishment in anuran eggs, and provide methods which should be useful for studies on primary embryonic induction.
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